Online Report: Lab Report: This report will encompass the last three (3) labs: protein expression, purification, and characterization. 1,000 words total – Since this is online – you must be efficient with your words! Make them count! Title: think of something semi-creative Intro words: this should have some background information on technique of protein production and purification. There is a good article here: Nat Methods. 2008 Feb;5(2):135-46. Protein production and purification. Also a good website: http://www.embl.de/pepcore/pepcore_services/index.html End with an objective statement where you briefly sum-up what you set out to do in this set of experiments. Include a brief hypothesis. M&M 250 words: be succinct, concise, and brief while allowing an informed reader to be able to replicate your work. Do not show calculations for prelabs. Results: show images of your results that you have already taken CAPTIONS: make sure you have good captions (captions don’t count against the word limit) Transformation plates images - Mention how many colonies you got. Flask images, purple pellet images (include the weight of your pellet) Images of Elution 1 and 2 in tubes, Nanodrop screen shots - Include yields from your Nanodrop spectrophotometry for your protein amounts. Show brief calculations for Beer’s law calculations. A=Ebc Protein Gel images, Image of Ladder from web as reference (molecular weight marker used – get from website of company) Discusssion Analyze your results, address sources of error, and answer any questions from the handout Q: Why do we use lysozyme? Why do we use Benzonase/Cyanase? Q: Explain what is in the sample after each step: Sample 1, 2, 3,4 ,5, and 6 Q: What is different about the Wash vs. the Elution buffer? Q: Briefly, explain how the HIS tag system works Conclusions Recap what was done State key finding(s) Address future directions/applications of this work to VDS research References (2-3)
Amron S
Aakash S
Andrew B
Brandon N
Michael T
Cody W
Divya G
Drew L
Daniel O
Fiona I
Ivy N
Inez C
James F
Alex H
Jennifer H
Janice K
Tom S
Kevan P
Kaarthik R
Ling W
Landon K
Max M
Mihir S
Ramya R
Ruifei W
Suman A
Stephanie B
Shreya B
Shane A
Shashika R
Sajan S2
Urvashi M
Zamaria J
Zach R
Online Report:
Lab Report:
This report will encompass the last three (3) labs: protein expression, purification, and characterization.
1,000 words total –
Since this is online – you must be efficient with your words! Make them count!
Title: think of something semi-creative
Intro words: this should have some background information on technique of protein production and purification.
There is a good article here: Nat Methods. 2008 Feb;5(2):135-46. Protein production and purification.
Also a good website: http://www.embl.de/pepcore/pepcore_services/index.html
End with an objective statement where you briefly sum-up what you set out to do in this set of experiments. Include a brief hypothesis.
M&M 250 words: be succinct, concise, and brief while allowing an informed reader to be able to replicate your work. Do not show calculations for prelabs.
Results: show images of your results that you have already taken
CAPTIONS: make sure you have good captions (captions don’t count against the word limit)
Transformation plates images - Mention how many colonies you got. Flask images, purple pellet images (include the weight of your pellet)
Images of Elution 1 and 2 in tubes, Nanodrop screen shots - Include yields from your Nanodrop spectrophotometry for your protein amounts.
Show brief calculations for Beer’s law calculations. A=Ebc
Protein Gel images, Image of Ladder from web as reference (molecular weight marker used – get from website of company)
Discusssion
Analyze your results, address sources of error, and answer any questions from the handout
Q: Why do we use lysozyme? Why do we use Benzonase/Cyanase?
Q: Explain what is in the sample after each step: Sample 1, 2, 3,4 ,5, and 6
Q: What is different about the Wash vs. the Elution buffer?
Q: Briefly, explain how the HIS tag system works
Conclusions
Recap what was done
State key finding(s)
Address future directions/applications of this work to VDS research
References (2-3)